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Gradient thermal cycler LMGT-B202 enabled with Peltier heating units which can process more than 99 cycles. It can accommodate 96×0.2 ml+77×0.5 ml sample in wells. User-friendly programming is achieved via 5.7inch LCD with graphical display and windows operations system. This device can be applied across a temperature range of 0 to 99.9 °C with maximum ramping rate is 4.5 °C/s.
Specifications
| Capacity | 96×0.2 ml+77×0.5 ml |
| Temperature Range | 0 to 99.9 °C |
| Maximum. Heating Rate | 4.5 °C/s |
| Maximum. Cooling Rate | 4 °C/s |
| Uniformity | ≤ ± 0.3 °C |
| Accuracy | ≤ ± 0.2 °C |
| Display Resolution | 0.1 °C |
| Temperature Control | Block\Tube |
| Ramping Rate Adjustable | 0.1 to 4.5 °C |
| Gradient Uniformity | ≤ ± 0.3 °C |
| Gradient Accuracy | ≤ ± 0.2 °C |
| Gradient Temperature. Range | 30 to 99.9 °C |
| Gradient Spread | 1 to 30 °C |
| Hot Lid Temperature | 30 to 110 °C |
| Hot Lid Height Adjustable | Step less Adjustable |
| Number Of Programs | 200 |
| Maximum .No.Of Segment | 9 |
| Maximum. No. Of Step | 9 |
| Maximum. No. Of Cycle | 99 |
| Increment/Decrement Time | 1 Second to 9 Min 59 Second |
| Increment/Decrement Temperature | 0.1 to 9.9 °C |
| Pause Function | Yes |
| Auto Data Protection | Yes |
| Graphical Display | Yes |
| Holding | 4 °C |
| LCD | 5.7-inch, 320×240 pels |
| Dimensions(L×W×H) | 392×262×252 mm |
| Weight | 10 kg |
| Power Consumption | 600 W |
| Power Supply | 85 to 264 V AC , 50 to 60 Hz |
Pricing
| Product | Price | Unit | Action |
|---|---|---|---|
| Gradient Thermal Cycler LMGT-B202 | $ 3712.5 | Pack of 1 |
Features
Uniform heating of the samples is achieved with Peltier heating units with more than 99 cycles
Maximum. heating Rate is 4.5 °C/s with temperature range of 0 to 99.9 °C
It has the capability to accommodate 96×0.2 ml, 96×0.2 ml+77×0.5 ml with moveable hot lid which can fit different sizes of tubes
All the operations are controlled by a computer software system which instrument PCR protocols
It has power of operation recovery option which helps in continuing the same command after a recovering from power cut
To satisfy the customers need it is installed with special feature like the user can add commands while the process is going on to encounter different variations
Applications
Used in Gene expression analysis, Pathogen detection, DNA methylation analysis, Genotyping and gene scanning, miRNA research etc.
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Frequently Asked Questions
1 What is a Gradient Thermal Cycler LMGT-B202?
Gradient Thermal Cycler LMGT-B202 is an apparatus designed for precise and efficient polymerase chain reaction (PCR) amplification.
2 How does a Gradient Thermal Cycler LMGT-B202 work?
A Gradient Thermal Cycler LMGT-B202 works on the principle of precise temperature cycling using Peltier elements to amplify DNA through the polymerase chain reaction (PCR).
3 How is the Gradient Thermal Cycler LMGT-B202 used in gene expression analysis?
Gradient Thermal Cycler LMGT-B202 amplifies specific RNA sequences converted to cDNA to measure gene expression levels.
4 What role does Gradient Thermal Cycler LMGT-B202 play in forensic and security industries?
Gradient Thermal Cycler LMGT-B202 amplifies trace DNA for human identification in criminal investigations or disaster victim identification.
5 Can Gradient Thermal Cycler LMGT-B202 help in agricultural biotechnology?
Gradient Thermal Cycler LMGT-B202 amplifies plant DNA to screen for desirable genetic traits or resistance genes.
6 Can Gradient Thermal Cycler LMGT-B202 detect leukemia-related mutations?
Gradient Thermal Cycler LMGT-B202 identifies fusion genes and mutations such as BCR-ABL in leukemia patients through PCR.
7 Is Gradient Thermal Cycler LMGT-B202 useful in monitoring minimal residual disease (MRD)?
Gradient Thermal Cycler LMGT-B202 amplifies specific cancer-related DNA or RNA to detect low levels of disease post-treatment.
8 Can Gradient Thermal Cycler LMGT-B202 assist in HLA typing for bone marrow transplants?
Gradient Thermal Cycler amplifies LMGT-B202 HLA gene regions for compatibility testing between donors and recipients.
9 Is Gradient Thermal Cycler LMGT-B202 used in studying blood cell lineage or differentiation?
Gradient Thermal Cycler amplifies LMGT-B202 amplifies lineage-specific gene markers to study hematopoietic differentiation.
10 What kind of maintenance does Advanced Gradient Thermal Cycler LMGT-B202 require?
Routine maintenance includes cleaning of the block and vents, inspection of the lid mechanism, and periodic functional checks to ensure optimal performance.
11 What is a Gradient Thermal Cycler?
A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.
12 How does Gradient Thermal Cycler work?
It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.
13 Can Gradient Thermal Cycler be used for multiplex PCR?
It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.
14 How does Gradient Thermal Cycler improve PCR efficiency in research labs?
It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.
15 Is Gradient Thermal Cycler useful in genotyping applications?
It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.
16 How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?
It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.
17 Is Gradient Thermal Cycler beneficial for forensic DNA analysis?
It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.
18 How does Gradient Thermal Cycler support microbiome or metagenomics studies?
It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.
19 Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?
It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.
20 How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?
It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.