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Gradient thermal cycler LMGT-B104 enabled with Peltier heating units which can process more than 100 cycles and store data in the internal flash memory. It can process 384 well sample. User-friendly programming is achieved via 8-inches TFT color touch-screen with graphical display and windows operations system. This device can be applied across a temperature range of 0 to 100 °C with maximum ramping rate is 4.5 °C/s. It is advantageous to use pre-installed 11 standard protocols and secure his data using password Login method. The files can be transferred via USB, printer support.
Specifications
| Capacity | 384 well |
| Temperature Range | 0 to 100 °C |
| Maximum. Heating Rate | 4.5 °C/s |
| Maximum. Cooling Rate | 4 °C/s |
| Uniformity | ≤ ± 0.2 °C |
| Accuracy | ≤ ± 0.1 °C |
| Display Resolution | 0.1 °C |
| Temperature Control | Block\Tube |
| Ramping Rate Adjustable | 0.1 to 4.5 °C |
| Gradient Uniformity | ≤ ± 0.2 °C |
| Gradient Accuracy | ≤ ± 0.2 °C |
| Gradient Temperature. Range | 30 to 100 °C |
| Gradient Spread | 1 to 30 °C |
| Hot Lid Temperature | 30 to 110 °C |
| Hot Lid Height Adjustable | Step less Adjustable |
| Number Of Programs | 10000 +(USB FLASH) |
| Maximum. No. Of Step | 30 |
| Maximum. No. Of Cycle | 100 |
| Increment/Decrement Time | 1 Second to 600 Second |
| Increment/Decrement Temperature | 0.1 to 10.0 °C |
| Pause Function | Yes |
| Auto Data Protection | Yes |
| Holding | 4 °C |
| Yes | |
| LAN To Computer | Yes |
| LCD | 8 inch,800×600 pels, 260000 colors |
| Interface | USB2.0 , LAN |
| Dimensions(L×W×H) | 390×270×255mm |
| Weight | 8.5 kg |
| Power Consumption | 600 W |
| Power Supply | 85 to 264 VAC , 47 to 63Hz |
Pricing
| Product | Price | Unit | Action |
|---|---|---|---|
| Gradient Thermal Cycler LMGT-B104 | $ 3787.5 | Pack of 1 |
Features
Uniform heating with 3 different temperature of the samples is achieved with Peltier heating units with more than 100 cycles
It is well built with anodizing technology with aluminum block treatment which makes the durable and corrosion resistant
Maximum heating rate is 4.5 °C/s with temperature range of 0 to 100 °C
It has the capability to accommodate 96×0.2ml+77×0.5ml with moveable hot lid which can fit different sizes of tubes
It has the internal flash memory to store the 10000 files data for analysis
The connectivity between computer is via USB port and mini printer and can store the GLP data after every step to get reliable results
All the operations are controlled by a computer software system which instrument PCR protocols and also has in built 11 standard protocols
It has power of operation recovery option which helps in continuing the same command after a recovering from power cut
The user can access to his individual experimental data by setting a password and data management system
To satisfy the customers need it is installed with special feature like the user can add sample while the process is going on to encounter different variations
The user will be sent notifications via email about the process to alert when operation ends
Applications
Used in Gene expression analysis, Pathogen detection, DNA methylation analysis, Genotyping and gene scanning, miRNA research etc
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Frequently Asked Questions
1 What is a Gradient Thermal Cycler?
A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.
2 How does Gradient Thermal Cycler work?
It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.
3 Can Gradient Thermal Cycler be used for multiplex PCR?
It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.
4 How does Gradient Thermal Cycler improve PCR efficiency in research labs?
It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.
5 Is Gradient Thermal Cycler useful in genotyping applications?
It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.
6 How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?
It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.
7 Is Gradient Thermal Cycler beneficial for forensic DNA analysis?
It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.
8 How does Gradient Thermal Cycler support microbiome or metagenomics studies?
It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.
9 Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?
It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.
10 How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?
It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.