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Gradient thermal cycler LMGT-B301 enabled with Peltier heating units which can process more than 100 cycles and store data in the internal flash memory. It can accommodate 96×0.2 ml (6 independent 16 well block) sample in wells. User-friendly programming is achieved via 8-inches TFT color touch-screen with graphical display and windows operations system. This device can be applied across a temperature range of 4 to 100 °C with maximum ramping rate is 6 °C/s. It is advantageous to the user to secure his data using password and user login method and use pre-installed 11 standard protocols. The files can be transferred via USB, printer support.
Specifications
| Capacity | 96×0.2 ml（6 independent 16 well block） |
| Temperature Range | 4 to 100 °C |
| Maximum. Heating Rate | 6 °C/s |
| Maximum. Cooling Rate | 4 °C/s |
| Uniformity | ≤ ± 0.3 °C |
| Accuracy | ≤ ± 0.2 °C |
| Display Resolution | 0.1 °C |
| Temperature Control | Block/ Tube |
| Ramping Rate Adjustable | 0.1 to 6 °C |
| Gradient Uniformity | 30 to 100 °C |
| Gradient Accuracy | Temperature difference of adjacent temperature zone is 0.1 to 5 °C. 6 temperature zones in total |
| Hot Lid Temperature | 30 to 110 °C |
| Hot Lid Height Adjustable | Step less Adjustable |
| Number Of Programs | 10000 +(USB FLASH) |
| Maximum. No. Of Step | 30 |
| Maximum. No. Of Cycle | 100 |
| Increment/Decrement Time | 1 Sec to 600 Sec |
| Increment/Decrement Temperature | 0.1 to 10.0 °C |
| Pause Function | Yes |
| Auto Data Protection | Yes |
| Holding | 4 °C |
| Touchdown Function | Yes |
| Long PCR Function | Yes |
| Lan To Computer | Yes |
| LCD | 8 inch，800×600 pels |
| Interface | USB2.0 , LAN |
| Dimensions(L×W×H) | 390×270×255 mm |
| Weight | 9 kg |
| Power Consumption | 600 W |
| Power Supply | 85 to 264 V AC , 47 to 63 Hz |
Pricing
| Product | Price | Unit | Action |
|---|---|---|---|
| Gradient Thermal Cycler LMGT-B301 | $ 4225 | Pack of 1 |
Features
Uniform heating with 3 different temperature of the samples is achieved with Peltier heating units with more than 100 cycles
It is well built with anodizing technology using aluminum block treatment which makes the durable and corrosion resistant
Maximum. heating Rate is 4.5 °C/s with temperature range of 0 to 100 °C
It has the capability to accommodate 96×0.2 ml(6 independent 16 well block) with moveable hot lid which can fit different sizes of tubes
It has the internal flash memory to store the 10000 files data for analysis
The connectivity between computer is via USB port and mini printer and can store the GLP data after every step to get reliable results
All the operations are controlled by a computer software system which instrument PCR protocols and also has in built 11 standard protocols
It has power of operation recovery option which helps in continuing the same command after a recovering from power cut
The user can access to his individual experimental data by setting a password and data management system
To satisfy the customers need it is installed with special feature like the user can add sample while the process is going on to encounter different variations
The user will be sent notifications via email about the process to alert when operation ends
Applications
Used in Gene expression analysis, Pathogen detection, DNA methylation analysis, Genotyping and gene scanning, miRNA research etc
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Frequently Asked Questions
1 What is a Gradient Thermal Cycler LMGT-B301?
A Gradient Thermal Cycler LMGT-B301 is a PCR machine that enables precise DNA amplification with temperature gradient capability for optimizing reaction conditions.
2 How does a Gradient Thermal Cycler LMGT-B301 work?
A Gradient Thermal Cycler LMGT-B301 works on the principle of Peltier thermoelectric technology, which enables rapid and precise heating and cooling for DNA amplification during PCR.
3 Does Gradient Thermal Cycler LMGT-B301 support amplification of plant DNA from leaves or seeds?
Gradient Thermal Cycler LMGT-B301 is used in agricultural and genetic research for testing plant-based DNA.
4 Can Gradient Thermal Cycler LMGT-B301 be used to analyze DNA from marine organisms like fish or algae?
Gradient Thermal Cycler LMGT-B301 amplifies genetic material from marine samples for biodiversity and ecological studies.
5 Does Gradient Thermal Cycler LMGT-B301 support PCR of DNA extracted from coral samples?
Gradient Thermal Cycler LMGT-B301 supports coral DNA amplification for reef biodiversity and stress-response studies.
6 Can Gradient Thermal Cycler LMGT-B301 amplify DNA from protozoan parasites like Plasmodium (malaria)?
Gradient Thermal Cycler LMGT-B301 is suitable for detecting protozoan DNA such as Plasmodium, Giardia, and Leishmania.
7 Can Gradient Thermal Cycler LMGT-B301 amplify DNA from larvae or eggs of parasites using this cycler?
Gradient Thermal Cycler LMGT-B301 is capable of amplifying DNA from all life stages of parasites, including eggs and larvae.
8 Is Gradient Thermal Cycler LMGT-B301 suitable for non-invasive prenatal testing (NIPT) using maternal plasma?
Gradient Thermal Cycler LMGT-B301 amplifies cell-free fetal DNA from maternal blood for early genetic screening.
9 Is Gradient Thermal Cycler LMGT-B301 suitable for analyzing degraded DNA from old or molted feathers?
Gradient Thermal Cycler LMGT-B301 allows reliable amplification of degraded or low-yield DNA.
10 What kind of maintenance does Advanced Gradient Thermal Cycler LMGT-B202 require?
Routine maintenance includes regular cleaning of the block and exterior, proper ventilation, software updates, and annual calibration.
11 What is a Gradient Thermal Cycler?
A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.
12 How does Gradient Thermal Cycler work?
It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.
13 Can Gradient Thermal Cycler be used for multiplex PCR?
It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.
14 How does Gradient Thermal Cycler improve PCR efficiency in research labs?
It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.
15 Is Gradient Thermal Cycler useful in genotyping applications?
It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.
16 How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?
It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.
17 Is Gradient Thermal Cycler beneficial for forensic DNA analysis?
It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.
18 How does Gradient Thermal Cycler support microbiome or metagenomics studies?
It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.
19 Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?
It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.
20 How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?
It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.