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Gradient PCR Machine LMGP-504 works at 4 to 99.9°C, with rapid heating rate. Its built-in gradient calculator optimizes annealing temperatures, enhancing reproducibility. Its high-capacity storage system, secure user login, and password protection, safeguard data integrity. Its thermostatic incubation and uniform thermal dissipation ensure reliable performance in demanding laboratory environments. Our Gradient PCR Machine is ideal for molecular biology, genetic and pharmaceutical research.
Specifications
| Temperature Range | 4 to 99.9°C |
| Sample Capacity | 96 × 0.2 ml |
| Gradient Temperature Uniformity | ±0.3°C |
| Gradient Temperature Accuracy | ±0.3°C |
| Gradient Temperature Range | 30 to 99.9°C |
| Gradient Setting Range | 0.1 to 30°C |
| Maximum Heating Rate | 4.5°C/s |
| Maximum Cooling Rate | 4°C/s |
| Temperature Uniformity | ±0.25°C |
| Temperature Accuracy | ±0.20°C |
| Temperature Display Resolution | 0.1°C |
| Temperature Change Rate | 0.1 to 5.0°C |
| Hot Cover Temperature Range | 30 to 110°C |
| Insulation Temperature | 16°C |
| Temperature Increment/Decrement | -9.9°C to 9.9°C |
| Time Increment/Decrement | -599 to 599s |
| Single Step Time Range | 1s to 59m59s |
| Maximum Program Steps | 30 |
| Maximum Program Cycles | 99 to 599 |
| Program Storage Capacity | Greater than 100 programs |
| Power Supply | 100 to 240V, 50/60Hz |
| Dimensions | 390 × 270 × 255 mm |
| Net Weight | 8.5 kg |
Pricing
| Product | Price | Unit | Action |
|---|---|---|---|
| Gradient PCR Machine LMGP-504 | $ 8625 | Each of 1 |
Features
Block/Tube Temperature Control
Hot Lid Height Adjustment
Program Pause Function
8-inch LCD touch screen
High-Quality Aluminium Blocks
USB and LAN Connectivity
Applications
Gradient PCR Machine LMGP-504 is suitable for gene amplification, DNA analysis, enzyme digestion, ELISA assays, and genetic sequencing in research and diagnostics.
Selection Guide
Gradient PCR Machine LMGP-504 is an advanced thermal cycler best suited for research applications that require accurate temperature control and optimization. It is widely used in gene expression studies, mutation detection, primer testing, and complex DNA amplification protocols. With a sample capacity of 96 × 0.2 ml and a broad temperature range of 4°C to 99.9°C, it enables efficient gradient functionality, allowing researchers to test multiple annealing temperatures simultaneously. The machine supports both block and tube temperature control modes, ensuring flexibility for diverse experimental setups. Its consistent temperature accuracy and uniformity make it suitable for high-throughput and reproducible results. It is particularly beneficial in molecular biology, clinical diagnostics, and environmental research. Additionally, it may come with certifications such as ISO or CE, which assure users of its safety, quality, and performance standards, making it a reliable choice for research laboratories.
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Frequently Asked Questions
1 What is a Gradient PCR Machine LMGP-504?
A Gradient PCR Machine LMGP-504 is a laboratory instrument designed for amplifying DNA through the polymerase chain reaction (PCR) process.
2 How does Gradient PCR Machine LMGP-504 work?
Gradient PCR Machine LMGP-504 works by amplifying DNA through thermal cycling with adjustable gradient temperatures to optimize reaction conditions.
3 Can Gradient PCR Machine LMGP-504 be used for gene expression studies?
Gradient PCR Machine LMGP-504 is ideal for gene expression studies by enabling precise amplification of cDNA templates during RT-PCR experiments.
4 Is Gradient PCR Machine LMGP-504 suitable for detecting mutations in DNA?
Gradient PCR Machine LMGP-504 is commonly used for mutation detection through allele-specific PCR and SNP genotyping.
5 Can Gradient PCR Machine LMGP-504 be used in cloning workflows?
Gradient PCR Machine LMGP-504 is widely used in molecular cloning for amplifying gene inserts before ligation into vectors.
6 Can Gradient PCR Machine LMGP-504 be used for pathogen detection in clinical samples?
Gradient PCR Machine LMGP-504 is suitable for clinical diagnostics involving pathogen detection through DNA/RNA amplification.
7 Is Gradient PCR Machine LMGP-504 useful in forensic DNA analysis?
Gradient PCR Machine LMGP-504 is frequently used in forensic labs for DNA profiling and amplification of STR markers.
8 Can Gradient PCR Machine LMGP-504 be used for environmental DNA (eDNA) studies?
Gradient PCR Machine LMGP-504 is highly effective for amplifying environmental DNA samples for biodiversity and ecological monitoring.
9 How does Gradient PCR Machine LMGP-504 assist in vaccine research?
Gradient PCR Machine LMGP-504 aids vaccine research by amplifying antigen-coding sequences, verifying constructs, and validating target gene expression.
10 Can Gradient PCR Machine LMGP-504 be used in personalized medicine research?
Gradient PCR Machine LMGP-504 is commonly used in personalized medicine research for amplifying genetic markers related to drug response and disease susceptibility.
11 What is a Gradient PCR?
A Gradient PCR is an apparatus designed for identifying the optimal annealing temperature for a specific PCR reaction.
12 Is Gradient PCR faster than running multiple individual PCR reactions?
It can test multiple temperatures in one experiment thereby saving time and providing quick results.
13 Can Gradient PCR be used for different sets of primers?
It is used for testing different sets of primers to find the best annealing temperature for each set.
14 Is Gradient PCR suitable for all types of DNA?
It can be used with various types of DNA templates such as genomic DNA, plasmid DNA, and cDNA.
15 Can Gradient PCR help with low-abundance templates?
It can identify the best conditions for amplification of low-abundance templates thereby improving the chances of getting good results.
16 Can Gradient PCR be used for amplifying large DNA fragments?
It can be used to amplify large DNA fragments.
17 Can Gradient PCR be used for DNA amplification from environmental samples?
It can be used for amplifying DNA from environmental samples such as soil, water or biological samples.
18 Can Gradient PCR be used for diagnostic PCR assays?
It can be used for diagnostic PCR assays.