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Single Step Time

Temperature control mode

Temperature Range

Module Temperature Range

Sample Capacity

Gradient PCR

Gradient PCR boasts a compact, space-efficient design, setting it apart as a gene amplification device enriched with gradient capabilities stemming from conventional PCR instruments. Its user-friendly interface, highlighted by a 5-inch high-definition color touchscreen, facilitates intuitive operation. The instrument delivers precise temperature control with remarkable uniformity, ensuring rapid heating and cooling cycles. Enhanced data management is achieved through swift data transfer to USB flash drives and effortless program backups, elevating storage capabilities. This PCR system incorporates cutting-edge semiconductor refrigeration technology, a testament to its advanced engineering.

Gradient PCR LMGP-501

  • Temperature control mode: Block or tube
  • Module Temperature Range: 4 to 99.9°C
  • Timing Range: 1s to 99 min 59sec/infinite length
  • Module temperature accuracy: ±0.2°C
  • Module Temperature Uniformity: ±0.25°C

$ 2912.5 / Pack of 1

Gradient PCR Machine LMGP-502

  • Single Step Time: 1 to 59m59s
  • Temperature Range: 4 to 99.9℃
  • Sample Capacity: 0.2ml × 96
  • Gradient: 12
  • Gradient Temperature Uniformity: ±0.3℃

$ 3200 / Each of 1

Gradient PCR Machine LMGP-503
  • Single Step Time: 1 to 59m59s
  • Temperature Range: 4 to 99.9℃
  • Sample Capacity: 384 × 0.04ml
  • Gradient: 24
  • Gradient Temperature Uniformity: ±0.3℃

$ 7375 / Each of 1

Gradient PCR Machine LMGP-504
  • Temperature Range: 4 to 99.9°C
  • Sample Capacity: 96 × 0.2 ml
  • Gradient Temperature Uniformity: ±0.3°C
  • Gradient Temperature Accuracy: ±0.3°C
  • Gradient Temperature Range: 30 to 99.9°C

$ 8625 / Each of 1

Selection Guide for Gradient PCR 

When selecting Gradient PCR, customers should focus on its wide temperature range of 4 to 99.9°C, which supports a broad spectrum of PCR applications, including standard PCR, mutagenesis, and gene expression studies. The gradient capability enables the simultaneous testing of multiple annealing temperatures in a single run, making it suitable for protocol optimization. With a flexible single-step time range from 1 second to 59 minutes and 59 seconds, users can fine-tune each stage of the PCR cycle. The system supports both block and tube temperature control modes, enhancing precision and consistency. It also provides versatile sample capacity options, including 96 × 0.2 ml tubes and 384 × 0.04 ml plates, catering to both standard and high-throughput laboratory workflows. These features make the Gradient PCR a reliable and adaptable choice for research and diagnostic labs.

Frequently Asked Questions

1 What is a Gradient PCR?

A Gradient PCR is an apparatus designed for identifying the optimal annealing temperature for a specific PCR reaction.

2 Is Gradient PCR faster than running multiple individual PCR reactions?

It can test multiple temperatures in one experiment thereby saving time and providing quick results.

3 Can Gradient PCR be used for different sets of primers?

It is used for testing different sets of primers to find the best annealing temperature for each set.

4 Is Gradient PCR suitable for all types of DNA?

It can be used with various types of DNA templates such as genomic DNA, plasmid DNA, and cDNA.

5 Can Gradient PCR help with low-abundance templates?

It can identify the best conditions for amplification of low-abundance templates thereby improving the chances of getting good results.

6 Can Gradient PCR be used for amplifying large DNA fragments?

It can be used to amplify large DNA fragments.

7 Can Gradient PCR be used for DNA amplification from environmental samples?

It can be used for amplifying DNA from environmental samples such as soil, water or biological samples.

8 Can Gradient PCR be used for diagnostic PCR assays?

It can be used for diagnostic PCR assays.